Molecular genetic characterization and haplotype diversity of swine leukocyte antigen in Chinese Rongshui miniature pigs. Mol Immunol. 2019 Jun 6;112:215-222-最新论文-保定市金诺兽药研究所

科研进展

当前位置: 首页» 科研进展» 最新论文

最新论文

Molecular genetic characterization and haplotype diversity of swine leukocyte antigen in Chinese Rongshui miniature pigs. Mol Immunol. 2019 Jun 6;112:215-222

CaixiaGao  ChangXin  XiuyingWang  JinqiangQuan  ChangwenLi  JingyuWang  HongyanChen

 

Mol Immunol. 2019 Jun 6;112:215-222. doi: 10.1016/j.molimm.2019.05.009

 

Abstract

The Rongshui miniature pig is an important model animal for studying livestock disease prevention and control in China. The highly polymorphic swine leukocyte antigen (SLA) has been the focus of considerable interest because of the strong, reproducible associations between particular SLA haplotypes and infectious diseases. In this study, we identified 42 alleles at eight polymorphic SLA loci (SLA-1, SLA-3, SLA-2, SLA-6, DRA, DRB1, DQA, and DQB1) representing seven class I and six class II haplotypes using reverse transcription-polymerase chain reaction (RT-PCR) sequence-based typing and PCR-sequence specific primers in Rongshui miniature pigs. The official names were designated by the SLA Nomenclature Committee of the International Society for Animal Genetics. Seven class I haplotypes, Hp-5b.0, 86.0, 87.0, 88.0, 89.0, 90.0 and 91.0, and four class II haplotypes, Hp-0.18b, 0.19c, 0.41 and 0.47, had not previously been reported in other pig breeds. We also comprehensively analyzed the molecular genetic characterization and phylogenies of the identified alleles and the SLA haplotype diversity in Rongshui miniature pigs. SLA-1 and SLA-6 genes were under positive selection, while SLA-2 was under neutral selection, and the other five genes were under purifying selection. The highly polymorphic new alleles may be derived by nucleotide mutations, insertions and deletions, and fragment recombination, and alleles segregated based on sequence differences and peptide-binding motifs, rather than on pig breed. SLA haplotype diversity was generated by allele/gene conversion and recombination. These results will be helpful for elucidating the molecular genetic mechanisms influencing differential disease resistance among pigs with different SLA haplotypes.

Copyright ? 2019 Elsevier Ltd. All rights reserved.

 

KEYWORDS:

Diversity; Genetic characterization; Haplotype; Major histocompatibility complex; Rongshui miniature pig; Swine leukocyte antigen

扫一扫 关注我
网站首页 联系我们
TOP